耿灿茹，管骏涛，陆晓.成纤维细胞生长因子-2在等长收缩促进远隔缺血心肌侧支动脉生成中的作用[J].中华物理医学与康复杂志,2018,40(5):331-335
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| 成纤维细胞生长因子-2在等长收缩促进远隔缺血心肌侧支动脉生成中的作用 |
| Effects of fibroblast growth factor-2 on collateral artery formation in an ischemic myocardium promoted by isometric exercise |
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| DOI： |
| 中文关键词: 等长收缩 心肌缺血 侧支动脉生成 成纤维细胞生长因子 |
| 英文关键词: Isometric exercise Myocardial ischemia Collateral artery formation Fibroblast growth factors |
| 基金项目:国家自然科学基金（81472164） |
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| 中文摘要: |
| 目的 研究成纤维细胞生长因子-2（FGF-2）在等长收缩运动（IE）促进远隔缺血心肌侧支动脉生成中的作用。 方法 将24只雄性SD大鼠（200±20）g，按随机数字表法分为假手术组（SO组）、心肌缺血组（MI组）、等长收缩训练组（IE组）和FGF抑制训练组（Inhi-FGF组）。SO组大鼠连续2周皮下注射生理盐水；MI组大鼠连续2周皮下注射异丙肾上腺素［10 mg/（kg·d）］；IE组大鼠在MI组的基础上进行等长收缩运动训练；Inhi-FGF组大鼠在IE组的基础上进行芒柄花黄素灌胃［100 mg/（kg·d）］。等长收缩训练8周后取大鼠左心室心肌。采用微球法测定相对侧支循环血流量（RCBF），免疫组化法测定小动脉密度和平滑肌细胞数量，Western blot法测定FGF-2及其受体FGFR-1的蛋白含量。 结果 与SO组、MI组和Inhi-FGF组相比，IE组RCBF、小动脉密度、平滑肌细胞数量和FGF-2及FGFR-1的蛋白相对表达量均明显增加（P<0.01）。Inhi-FGF组小动脉密度、平滑肌细胞数量和FGF-2蛋白相对表达量均显著低于MI组（P<0.05），但Inhi-FGF组RCBF和FGFR-1蛋白相对表达量与MI组间差异无统计学意义（P>0.05）。RCBF与小动脉密度、小动脉密度与平滑肌细胞数量以及平滑肌细胞数量与FGF-2蛋白表达呈直线正相关（P<0.01）。 结论 IE可通过促进FGF-2及其受体的表达，从而促进远隔缺血心肌侧支动脉生成，改善血流灌注。 |
| 英文摘要: |
| Objective To investigate the effect of fibroblast growth factor-2 (FGF-2) on the formation of collateral arteries in the remote myocardium promoted by the isometric exercise (IE). Methods Twenty-four male Sprague-Dawley rats weighing (200±20)g were randomized into a sham operation group (SO),a myocardial ischemia group (MI), an isometric exercise group (IE) and an FGF inhibitor group (Inhi-FGF), each of 6. Rats in the SO group were injected with saline subcutaneously for 2 weeks while those of the MI group were being injected with 10 mg/（kg·d） of isoproterenol subcutaneously to induce myocardial ischemia. Rats in group IE accepted isometric exercise and the same injections as group MI, while those in group Inhi-FGF were also given 100 mg/（kg·d） of formononetin intragastrically in addition to the group IE treatment. After 8 weeks of IE the myocardium of the rats′ left ventricles was resected. The relative collateral blood flow (RCBF) was measured using the microsphere method. The artery density and the number of smooth muscle cells were evaluated using immunohistochemical analysis. Western blotting was performed to assay the levels of FGF-2 protein and its receptor FGFR-1. Results Compared to the SO, MI and Inhi-FGF groups, significant increases in the RCBF, artery density, the number of smooth muscle cells and the relative levels of FGF-2 protein and FGFR-1 were observed in group IE. In the Inhi-FGF group the artery density, the number of smooth muscle cells and the relative protein level of FGF-2 were significantly lower than those of the MI group, but there were no significant differences in the RCBF or FGFR-1 levels between the two groups. The artery density had a positive linear correlation with the number of smooth muscle cells. The RCBF correlated with the artery density, the number of smooth muscle cells and the FGF-2 protein levels. Conclusion IE can promote the expression of FGF-2 and its acceptor, resulting in the formation of collateral arteries and better blood perfusion of an ischemic myocardium. |
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