郭君，李哲，范家宏，郭钢花，李晓丽，关晨霞，乐琳.不同间歇时间磁刺激对星形胶质细胞迁移能力的影响及机制分析[J].中华物理医学与康复杂志,2018,40(5):325-330
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| 不同间歇时间磁刺激对星形胶质细胞迁移能力的影响及机制分析 |
| The effects of the inter-stimulus interval in magnetic stimulation on astrocyte migration and its mechanism |
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| DOI： |
| 中文关键词: 磁刺激 星形胶质细胞 PEA-15 细胞迁移 |
| 英文关键词: Magnetic stimulation Astrocytes PEA-15 Migration |
| 基金项目:河南省基础与前沿技术研究计划项目(142300410035) |
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| 中文摘要: |
| 目的 探讨不同间歇时间磁刺激对星形胶质细胞迁移能力的影响及相关机制。 方法 将传代星形胶质细胞分为对照组、1 s间歇组、5 s间歇组和10 s间歇组，分别给予相应间歇时间磁刺激，观察不同间歇时间磁刺激对星形胶质细胞迁移能力的影响；星形胶质细胞在磁刺激作用下，采用PEA-15磷酸化阻滞剂Bis I、细胞外调节蛋白激酶（ERK1/2）阻滞剂U0126处理细胞，采用Transwell实验检测星形胶质细胞迁移能力，采用Western blot技术检测pPEA-15和pERK1/2表达。 结果 1 s间歇时间磁刺激可明显增强星形胶质细胞迁移能力，促进PEA-15及ERK1/2磷酸化，并提高基质金属蛋白酶-9（MMP-9）表达；加入Bis I可降低ERK1/2磷酸化水平及MMP-9表达，减弱磁刺激对星形胶质细胞的促迁移作用；经U0126试剂处理后，发现磁刺激对星形胶质细胞的促迁移作用显著下降。 结论 1 s间歇时间磁刺激能促进星形胶质细胞PEA-15磷酸化，提高ERK1/2磷酸化水平，进而增强下游蛋白MMP-9表达，从而促进星形胶质细胞迁移。 |
| 英文摘要: |
| Objective To examine the effect of the inter-stimulus interval (ISI) in magnetic stimulation (MS) on astrocyte migration and its related mechanism. Methods Cultured astrocytes were treated with intermittent MS with intervals of 1, 5 and 10 seconds. The PEA-15 inhibitor BisI (10 μmol/ml) and the ERK1/2 inhibitor U0126 (10 μmol/ml) were administered and cell migration assays evaluated the astrocytes′ migration. The expression of phosphorylated ERK1/2 and PEA-15 was detected using Western blotting. Results The 1 second interval significantly facilitated astrocyte migration, the phosphorylation of PEA-15 and ERK1/2, and the expression of MMP-9 (browse matrix metalloproteinase-9). The addition of Bis I significantly reduced the production of phosphorylated ERK1/2 and MMP-9, as well as astrocyte migration induced by MS. In addition, pretreatment with U0126 also significantly decreased the astrocyte migration induced by MS. Conclusion 1s-ISI MS can induce PEA-15 activation and subsequently lead to ERK1/2 phosphorylation and upregulation of MMP-9, which may contribute to the migration of astrocytes. |
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