文章摘要
张真,张兆辉.电针联合经颅磁刺激对脑梗死大鼠学习记忆能力的影响及相关机制分析[J].中华物理医学与康复杂志,2018,40(4):257-261
电针联合经颅磁刺激对脑梗死大鼠学习记忆能力的影响及相关机制分析
The effect of electroacupuncture combined with repetitive transcranial magnetic stimulation on rats′ learning and memory after middle cerebral artery occlusion and reperfusion
  
DOI:
中文关键词: 脑梗死  电针  重复经颅磁刺激  水迷宫  生长相关因子-43
英文关键词: Cerebral ischemia  Electroacupuncture  Transcranial magnetic stimulation  Growth-associated protein 43
基金项目:
作者单位
张真,张兆辉 430060 武汉武汉大学人民医院神经内科 
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中文摘要:
      目的 观察电针联合重复经颅磁刺激对脑梗死大鼠行为学、脑梗死体积及学习记忆能力的影响,并探讨相关作用机制。 方法 采用随机数字表法将40只雄性SD大鼠分为正常组、假手术组、模型组及治疗组,每组10只大鼠。采用Zea-Longa线栓法将模型组及治疗组大鼠制成大脑中动脉栓塞/再灌注(MCAO/R)模型,治疗组大鼠于制模后24h给予电针及重复经颅磁刺激治疗。连续治疗14d后,采用修正版神经功能缺损评分(mNSS)检查各组大鼠神经功能,采用TTC法检测脑梗死面积,采用Morris水迷宫检测大鼠学习记忆能力,采用免疫组化法检测大鼠脑梗死周边区生长相关蛋白-43(GAP-43)表达。 结果 与模型组比较,治疗组大鼠神经功能缺损评分[(2.8±0.84)分]明显降低(P<0.05),脑梗死体积[(41.9±3.69)mm3]显著缩小(P<0.05),逃避潜伏期[(20.84±1.35)s]明显缩短(P<0.05),脑梗死周边区GAP-43表达[(22.98±1.24)个/每高倍镜视野]明显增多(P<0.05)。 结论 电针结合重复经颅磁刺激能促进MCAO/R模型大鼠神经功能及学习记忆能力提高,其治疗机制可能与促进脑梗死周边区GAP-43表达进而加速神经组织重塑有关。
英文摘要:
      Objective To study the effect of electroacupuncture combined with repetitive transcranial magnetic stimulation on the infarct volume of rats with cerebral infarction as well as their behavior and their learning and memory ability. Methods Forty male Sprague-Dawley rats were randomly divided into a normal group, a sham-operation group, a model group and a treatment group, each of 10. Focal cerebral ischemia and reperfusion was modeled in the latter two groups using thread occlusion of the middle cerebral artery. The treatment group was treated for 14 days with electroacupuncture combined with repetitive transcranial magnetic stimulation, started 24 hours after the operation. The animals′ neurological functioning was quantified using modified neurological severity scores. The area of cerebral infarct was measured using 5-triphenyltetrazolium chloride staining. The rats′ learning and memory abilities were assessed using a Morris water maze and the expression of growth-associated protein 43 (GAP-43) in the peripheral area of the cerebral infarction. Results The average neurological deficit score (2.8±0.84) was significantly lower in the treatment group than in the model group, the average infarct volume was smaller and the escape latency was less. In the treatment group the average expression of GAP-43 was greater than in the model group. Conclusion Electroacupuncture combined with repetitive transcranial magnetic stimulation can promote the expression of GAP-43 in the peripheral area of a cerebral infarct, promoting neuronal remodeling and thereby improving neurological functioning as well as learning and memory ability.
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