邓钰，王强，马健，孙丹乔，王欣欣.运动训练联合氟西汀治疗对卒中后抑郁大鼠抑郁行为及海马区BDNF和TrkB蛋白的影响[J].中华物理医学与康复杂志,2018,40(3):161-166
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| 运动训练联合氟西汀治疗对卒中后抑郁大鼠抑郁行为及海马区BDNF和TrkB蛋白的影响 |
| Exercise combined with fluoxetine medication can relieve post-stroke depression |
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| DOI： |
| 中文关键词: 脑卒中 抑郁 氟西汀 运动训练 行为学 |
| 英文关键词: Stroke Depression Fluoxetine Exercise |
| 基金项目:山东省自然科学基金（ZR2014JL057） |
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| 中文摘要: |
| 目的 观察运动训练联合氟西汀治疗对卒中后抑郁大鼠抑郁程度及海马区BDNF、TrkB蛋白表达的影响，并探讨联合治疗改善卒中后抑郁程度可能的机制。 方法 取48只雄性成年Wister大鼠，随机将大鼠分为假手术组、模型组、氟西汀组和联合治疗组，每组12只。除假手术组外，其余3组均采用线栓法建立左侧大脑中动脉（MCAO)模型并给予慢性不可预知性应激刺激（CUMS）以此来建立卒中后抑郁大鼠模型（PSD模型），而假手术组仅分离颈总、颈内、颈外动脉，不作插线处理，也不给予CUMS。造模成功后，氟西汀组给予氟西汀治疗，联合治疗组接受运动训练及氟西汀治疗，共为期28d，模型组不给予任何训练及药物治疗。四组大鼠将分别于PSD模型成功后1d、14d及28d进行行为学实验（蔗糖偏好试验、强迫游泳试验、体质量检测），并于测试后取出相应时间点的脑组织，采用western blot法检测左侧海马区BDNF及TrkB的表达变化情况。 结果 模型组与假手术组比较,各项指标差异均有统计学意义（P<0.05）。造模后第14天和第28天，氟西汀组和联合治疗组大鼠强迫游泳试验中的静止不动时间明显低于同一时间点的模型组(P<0.05)，而蔗糖偏好试验的蔗糖偏好度和体重均显著高于模型组(P<0.05)；海马区BDNF及TrkB表达亦较模型组显著增强(P<0.05)。同一时间点比较，联合治疗组较氟西汀组强迫游泳试验中的静止不动时间明显减少，且蔗糖偏好度和体重显著增加，海马区BDNF、TrkB表达亦显著高于氟西汀组(P<0.05）。 结论 运动训练联合氟西汀治疗可较好改善卒中后抑郁大鼠的抑郁状况。 |
| 英文摘要: |
| Objective To observe the effect of exercise combined with fluoxetine medication on depression and the expression of BDNF and TrkB protein in the hippocampus and to explore possible mechanisms. Methods Forty-eight male Wister rats were randomly divided into a sham operation group, a model group, a fluoxetine group and a combined treatment group, each of 10. A model of post-stroke depression was induced in all except the rats in the sham operation group through occlusion of the middle cerebral artery using the intraluminal tread method followed by imposing chronic but unpredictable stress. The model and sham operation groups were not given any training or medication. The fluoxetine group was treated with fluoxetine, while the combined treatment group received exercise training and the fluoxetine treatment at the same time for 28 days. After one, 14 and 28 days the four groups were given the sucrose preference test, a forced swimming test and were weighed. Brain tissue was resected to detect the expression of BDNF and TrkB in the left hippocampus using western blotting. Results There were significant differences between the model group and the sham operation group in all measurements. After both 14 days and 28 days of treatment, the average immobility times in the fluoxetine and combined treatment groups was significantly shorter than that in the model group, while their sucrose preference, average body weight and the expression of BDNF and TrkB in the left hippocampus were all significantly higher. The same differences were observed between the combined group and the fluoxetine group. Conclusion Exercise combined with fluoxetine medication can relieve depression, at least in rats. |
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