关晨霞，郭钢花，赵玉敏，李哲.磁刺激通过星形胶质细胞磷酸化蛋白影响星形胶质细胞迁移[J].中华物理医学与康复杂志,2018,40(2):81-86
扫码阅读全文
|
| 磁刺激通过星形胶质细胞磷酸化蛋白影响星形胶质细胞迁移 |
| Effects of magnetic stimulation on astrocyte migration |
| |
| DOI： |
| 中文关键词: 磁刺激 星形胶质细胞 磷酸化蛋白 细胞转染 |
| 英文关键词: Magnetic stimulation Astrocytes Phosphorylated protein Cell transfection |
| 基金项目:河南省科技厅基础研究计划项目（1423004135） |
|
| 摘要点击次数: 3314 |
| 全文下载次数: 3970 |
| 中文摘要: |
| 目的 研究1.0Hz 60%最大刺激强度的磁刺激通过调控星形胶质细胞磷酸化蛋白（PEA-15）对星形胶质细胞迁移的影响。 方法 取第3～4代体外分离培养的大鼠星形胶质细胞，分为对照组、转染组、磁刺激组和转染+磁刺激组。对照组进行阴性siRNA转染，转染组应用化学合成的siRNA进行脂质体瞬时转染，干扰PEA-15的蛋白表达；磁刺激组的星形胶质细胞在铺板24h后接受60%最大强度磁刺激；转染+磁刺激组进行PEA-15的siRNA转染，并给予60%最大强度磁刺激。用细胞划痕试验检测星形胶质细胞的迁移程度，用免疫印迹试验检测PEA-15的蛋白表达和磷酸化水平变化。 结果 ①PEA-15 siRNA的转染效果明显，Western Blot检测与对照组比较，PEA-15的蛋白表达明显降低。②体外划痕实验中,转染组、磁刺激组、转染+磁刺激组的细胞迁移面积分别与对照组比较，其星形胶质细胞迁移程度增加，差异均有统计学意义（P<0.05）。③与对照组相比，磁刺激组的PEA-15磷酸化明显增加。 结论 干扰PEA-15表达后的星形胶质细胞迁移增加明显；磁刺激可通过增强PEA-15磷酸化促进星形胶质细胞的迁移。 |
| 英文摘要: |
| Objective To study the effect of the transcranial magnetic stimulation on the migration of phosphoprotein-enriched astrocytes-15kDa (PEA-15). Methods Third or fourth generation rat astrocytes cultured in vitro were divided into a control group, a transfected group, a magnetic stimulation group and a transfected + magnetic stimulation group. The control group was undergone transfect of negative siRNA. In transfected group and the transfected + magnetic stimulation group the liposome in the astrocyte was transfected instantly with chemically synthesized PEA-15 siRNA, so as to interfere with the expression of PEA-15 protein. Magnetic stimulation was applied to both tranfected and transfected + magnetic stimulation groups 24 h after plating of astrocytes at 1 Hz and 60% the maximal output of the stimulator. Cell scratch tests were used to assess the astrocytes′ migration, and Western blotting was applied to detect the expression of PEA-15 and protein phosphorylation. Results Compared with the control group, the expression of PEA-15 protein decreased significantly in the transfected groups. The cell migration in the transfected group, the magnetic stimulation group, and the transfected + magnetic stimulation group was significantly greater than in the control group. Compared with the control group, the phosphorylation of PEA-15 increased significantly in the magnetic stimulation group. Conclusion When PEA-15 expression is interfered with, the migration of astrocytes increases significantly. Magnetic stimulation may promote the migration of astrocytes by enhancing PEA-15 phosphorylation. |
|
查看全文
查看/发表评论 下载PDF阅读器 |
| 关闭 |
