文章摘要
关晨霞,郭钢花,赵玉敏,李哲.磁刺激通过星形胶质细胞磷酸化蛋白影响星形胶质细胞迁移[J].中华物理医学与康复杂志,2018,40(2):81-86
磁刺激通过星形胶质细胞磷酸化蛋白影响星形胶质细胞迁移
Effects of magnetic stimulation on astrocyte migration
  
DOI:
中文关键词: 磁刺激  星形胶质细胞  磷酸化蛋白  细胞转染
英文关键词: Magnetic stimulation  Astrocytes  Phosphorylated protein  Cell transfection
基金项目:河南省科技厅基础研究计划项目(1423004135)
作者单位
关晨霞,郭钢花,赵玉敏,李哲 450052 郑州河南郑州大学第五附属医院康复医学科 
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中文摘要:
      目的 研究1.0Hz 60%最大刺激强度的磁刺激通过调控星形胶质细胞磷酸化蛋白(PEA-15)对星形胶质细胞迁移的影响。 方法 取第3~4代体外分离培养的大鼠星形胶质细胞,分为对照组、转染组、磁刺激组和转染+磁刺激组。对照组进行阴性siRNA转染,转染组应用化学合成的siRNA进行脂质体瞬时转染,干扰PEA-15的蛋白表达;磁刺激组的星形胶质细胞在铺板24h后接受60%最大强度磁刺激;转染+磁刺激组进行PEA-15的siRNA转染,并给予60%最大强度磁刺激。用细胞划痕试验检测星形胶质细胞的迁移程度,用免疫印迹试验检测PEA-15的蛋白表达和磷酸化水平变化。 结果 ①PEA-15 siRNA的转染效果明显,Western Blot检测与对照组比较,PEA-15的蛋白表达明显降低。②体外划痕实验中,转染组、磁刺激组、转染+磁刺激组的细胞迁移面积分别与对照组比较,其星形胶质细胞迁移程度增加,差异均有统计学意义(P<0.05)。③与对照组相比,磁刺激组的PEA-15磷酸化明显增加。 结论 干扰PEA-15表达后的星形胶质细胞迁移增加明显;磁刺激可通过增强PEA-15磷酸化促进星形胶质细胞的迁移。
英文摘要:
      Objective To study the effect of the transcranial magnetic stimulation on the migration of phosphoprotein-enriched astrocytes-15kDa (PEA-15). Methods Third or fourth generation rat astrocytes cultured in vitro were divided into a control group, a transfected group, a magnetic stimulation group and a transfected + magnetic stimulation group. The control group was undergone transfect of negative siRNA. In transfected group and the transfected + magnetic stimulation group the liposome in the astrocyte was transfected instantly with chemically synthesized PEA-15 siRNA, so as to interfere with the expression of PEA-15 protein. Magnetic stimulation was applied to both tranfected and transfected + magnetic stimulation groups 24 h after plating of astrocytes at 1 Hz and 60% the maximal output of the stimulator. Cell scratch tests were used to assess the astrocytes′ migration, and Western blotting was applied to detect the expression of PEA-15 and protein phosphorylation. Results Compared with the control group, the expression of PEA-15 protein decreased significantly in the transfected groups. The cell migration in the transfected group, the magnetic stimulation group, and the transfected + magnetic stimulation group was significantly greater than in the control group. Compared with the control group, the phosphorylation of PEA-15 increased significantly in the magnetic stimulation group. Conclusion When PEA-15 expression is interfered with, the migration of astrocytes increases significantly. Magnetic stimulation may promote the migration of astrocytes by enhancing PEA-15 phosphorylation.
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