唐雷，张红星，徐派的，康朝霞.电针激活磷酸化AMPKα通路对功能性消化不良大鼠mTOR基因表达的影响[J].中华物理医学与康复杂志,2018,40(11):809-813
扫码阅读全文
|
| 电针激活磷酸化AMPKα通路对功能性消化不良大鼠mTOR基因表达的影响 |
|
| |
| DOI： |
| 中文关键词: 电针 功能性消化不良 哺乳动物雷帕霉素靶蛋白 腺苷酸激活蛋白激酶α |
| 英文关键词: Electroacupuncture Functional dyspepsia mTOR AMPKα |
| 基金项目:国家自然科学基金资助项目（81473790，81704178）；武汉市卫计委资助项目（WZ16A03） |
|
| 摘要点击次数: 2910 |
| 全文下载次数: 3680 |
| 中文摘要: |
| 目的 探讨电针激活AMPKα通路治疗功能性消化不良（FD）的可能机制。 方法 随机挑选10只SD大鼠纳入正常组，余大鼠采用夹尾刺激法配合不规则饮食构建FD大鼠模型，将造模成功FD大鼠随机分为模型组、电针组，每组10只大鼠。电针组大鼠连续给予10 d电针刺激；正常组与模型组大鼠不给予电针等特殊干预。观察各组大鼠日常表现，并采用Western blot技术检测各组大鼠胃及小肠组织中p-AMPKα、p-TSC2及Rheb蛋白表达，同时采用RT-PCR技术检测各组大鼠胃及小肠中mTOR mRNA表达。 结果 Western blot检查结果显示，与正常组比较，模型组大鼠胃及小肠中p-AMPKα、p-TSC2蛋白表达明显降低（P<0.05），Rheb蛋白表达显著升高(P<0.05)；与模型组比较，电针组大鼠p-AMPKα、p-TSC2表达显著升高（P<0.05），Rheb蛋白表达明显降低(P<0.05)。RT-PCR检查结果显示，与正常组比较，模型组大鼠胃及小肠中mTOR mRNA表达显著上调(P<0.05)；与模型组比较，电针组大鼠mTOR mRNA表达显著下调(P<0.05)。 结论 电针能激活AMPKα通路，上调该通路相关蛋白p-TSC2表达，降低Rheb蛋白表达，从而下调mTOR基因转录，达到治疗FD目的。 |
| 英文摘要: |
| Objective To investigate how electroacupuncture activates the AMPKα pathway in the treatment of functional dyspepsia (FD). Methods Tail clipping stimulation was combined with an irregular diet to induce FD in 20 Sprague-Dawley rats. They were randomly divided into a model group and an electroacupuncture group, each of 10. Ten others had no FD induced and formed a normal group. The rats in the electroacupuncture group were given 10 days of electroacupuncture treatment, while those in the normal and model groups were restrained and fixed like those in the electroacupuncture group, but not given electroacupuncture. The expression of phosphorylated adenosine monophosphate-activated protein kinase alpha (p-AMPKα), phosphorylated tuberin (p-TSC2) and Rheb protein in the stomachs and small intestines of each group were detected using the western blotting. Levels of mTOR mRNA were quantified using the reverse-transcription polymerase chain reaction (RT-PCR). Results The western blotting results showed that compared with the normal group, the expression levels of p-AMPKα and p-TSC2 in the stomachs and small intestines of the model group decreased significantly, while that of Rheb protein increased significantly. Compared with the model group, the expression of p-AMPKα and p-TSC2 increased significantly after the electroacupuncture treatment, while that of Rheb protein decreased significantly. According to the RT-PCR results, the expression of mTOR mRNA in the stomachs and small intestines of the model group was up-regulated significantly compared with the normal group. Compared with the model group, expression of mTOR mRNA in the electroacupuncture group was significantly down-regulated. Conclusion Electroacupuncture can activate the AMPKα pathway, up-regulate the expression of the related protein p-TSC2, and decrease the expression of Rheb protein, thereby down-regulating the transcription of the mTOR gene. That would serve to treat FD. |
|
查看全文
查看/发表评论 下载PDF阅读器 |
| 关闭 |
