周文，陈艳华，肖登.脉冲电磁场对去卵巢大鼠Runt相关转录因子2表达的影响[J].中华物理医学与康复杂志,2017,39(9):651-654
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| 脉冲电磁场对去卵巢大鼠Runt相关转录因子2表达的影响 |
| The influence of a pulsed magnetic field on the expression of runt-related transcription factor 2 in rats after ovary resection |
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| DOI： |
| 中文关键词: 骨质疏松 脉冲电磁场 Runt相关转录因子2 |
| 英文关键词: Osteoporosis Pulsed electromagnetic fields Runx2 gene |
| 基金项目:重庆市教育委员会科学技术研究项目（KJ1500240） |
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| 中文摘要: |
| 目的 观察脉冲电磁场(PEMFs)对去卵巢大鼠骨细胞Runt相关转录因子2(Runx2)表达的影响，并探讨PEMFs治疗骨质疏松可能的作用机制。 方法 将60只Sprague Dawley（SD）雌性大鼠按随机数字表法分成假手术对照组（Sham组）、去卵巢组（OVX组）、去卵巢+阿伦磷酸钠药物治疗组（ALN组）、去卵巢+脉冲电磁场治疗组（PEMFs组），每组15只大鼠，按文献方法，Sham组切除双侧卵巢周围脂肪组织，其余各组大鼠切除双侧卵巢制成骨质疏松模型。手术结束后第30天，ALN组大鼠开始给予阿伦磷酸钠灌胃，PEMFs治疗组大鼠予以PEMFs治疗，Sham组和OVX组不干预。各组动物均于干预30d后采用腹腔注射戊巴比妥钠处死，取股骨行骨密度检测；采用Western bloting检测Runx2蛋白表达量；采用q-PCR检测Runx2 mRNA表达量。 结果 干预30d后，PEMFs组的骨密度值、Runx2蛋白表达水平和Runx2 mRNA表达水平较OVX组均有明显升高，差异有统计学意义（P<0.05）；但PEMFs组的骨密度值、Runx2蛋白表达水平和Runx2 mRNA表达水平与ALN组比较，组间差异均无统计学意义(P>0.05)。 结论 PEMFs可上调去卵巢SD雌性大鼠的Runx2蛋白和mRNA表达水平，可能是PEMFs治疗骨质疏松的机制之一。 |
| 英文摘要: |
| Objective To observe the influence of a pulsating electromagnetic field (PEMF) on the expression of runt-related transcription factor 2 (Runx2) in ovariectomized rats so as to explore the possibility of using PEMFs to treat osteoporosis. Methods Sixty female Sprague-Dawley rats were randomly divided into four groups of 15-a control group (Sham group), an ovary resection group (OVX group), a resection group treated with alendronate sodium (OVX+ALN group) and a resection group exposed to a PEMF (PEMFs group). Both ovaries were resected to induce osteoporosis, except in the sham group where the adipose tissues around the ovaries were cut bilaterally without resection. After the operation, the ALN group were given 2 mg/kg of alendronate by gavage daily for 30 days, while the PEMFs group was exposed to a 3.8 mT electromagnetic field pulsing at 8 Hz for 40 min twice daily for 30 days. There was no intervention in the other two groups. The animals were sacrificed using intra-peritoneal injection of sodium pentobarbital and the density of their femurs was measured. The expression of Runx2 protein and Runx2 mRNA were detected using western blotting and quantitative PCR. Results The average bone density, Runx2 protein level and Runx2 mRNA level of the PEMFs group were all significantly higher than those of the OVX group, but there was no significant difference between the PEMFs group and ALN group averages. Conclusion PEMF exposure can upregulate the expression of Runx2 protein and Runx2 mRNA in female rats after ovary resection. This may be one of the mechanisms by which PEMFs treat osteoporosis. |
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