梁顺利，吴忧，张荣博，章水晶，徐彬.运动训练对脑出血大鼠LINGO-1表达的影响[J].中华物理医学与康复杂志,2017,39(9):641-645
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| 运动训练对脑出血大鼠LINGO-1表达的影响 |
| Effects of exercise on the expression of the LINGO-1 gene in rats after intra-cerebral hemorrhage |
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| DOI： |
| 中文关键词: 脑出血 运动 神经再生 |
| 英文关键词: LINGO-1 gene Cerebral hemorrhage Exercise Nerve regeneration |
| 基金项目:浙江省医药卫生科技计划项目（2014KYB179） |
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| 中文摘要: |
| 目的 探讨运动训练对脑出血大鼠神经功能恢复和脑内LINGO-1表达的影响。 方法 选取72只SD大鼠按随机数字表法分为假手术组、模型组和运动训练组，每组24只，各组再按观察时间的不同随机分为1、3、7和14d四个时间点，每个时间点6只大鼠。制备脑出血模型，运动训练组采用跑台跑步法进行跑步运动训练。各组大鼠采用Longa评分法评价神经功能缺损程度，RT-PCR法检测LINGO-1 mRNA的表达，Western blot法检测LINGO-1蛋白的表达。 结果 假手术组神经功能损伤轻，Longa评分0或1分，各时间点LINGO-1 mRNA和蛋白表达均仅少量表达。模型组Longa评分7d最高达3分，14d出现下降到2分；LONG-1 mRNA 3d表达最高达0.737±0.041，7d出现下降为0.429±0.035；LONG-1蛋白7d到高峰达1.335±0.393，14d出现下降为1.225±0.424。运动训练组在14d Longa评分为1分，较模型组下降（P<0.05）；在7d和14d LINGO-1 mRNA表达分别为0.257±0.042和0.137±0.035，较模型组下降（P<0.05）；在14d LINGO-1蛋白表达为0.663±0.276，较模型组下降（P<0.05）；且各时间点LINGO-1蛋白的表达与Longa评分成正相关（r1d=0.837,P1d=0.036;r3d=0.845,P3d=0.047;r7d=0.891,P7d=0.029;r14d=0.926,P14d=0.018）。 结论 运动训练可下调LINGO-1 mRNA和蛋白的表达，促进脑出血后神经功能恢复。 |
| 英文摘要: |
| Objective To explore the effect of exercise on the expression of the LINGO-1 gene after intra-cerebral hemorrhage (ICH). Methods Seventy-two Sprague-Dawley rats were randomly divided into a sham operation group, a control group and an exercise training group, each of 24. The three groups were further divided into 1 d, 3 d, 7 d and 14 d groups. ICH was induced using intra-parenchymal injection of autologous blood. The exercise training group was then forced to exercise on a treadmill. Any recovery of neurological functional was evaluated using Longa scoring, and the expression of LINGO-1 mRNA and protein were detected using the reverse transcription-polymerase chain reaction and western blotting. Results The sham operation group displayed no obvious neurological deficiency, with little expression of LINGO-1 mRNA or protein at any time point. The control group′s average Longa score reached a maximum of 3 seven days after the operation, decreasing to 2 after another 7 days. The average expression of LINGO-1 mRNA and protein in that group peaked at 1.335±0.393 three days after the ICH, then decreased to 0.429±0.035 on the 7th day and 0.371±0.038 on the 14th day. In the exercise training group the average Longa score on the 7th day was 2, the average LINGO-1 mRNA level was 0.257±0.042 and the average protein level was 1.142±0.287, all significantly lower than in the control group. Moreover, in the exercise group there was a significant positive correlation between LINGO-1 protein expression and the Longa scores. Conclusion Exercise can decrease the expression of LINGO-1 mRNA and protein and promote the recovery of neural function after ICH. |
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