韩德雄，刘喆，张莺，刘婧.电针对慢性脑低灌注大鼠认知功能及海马JAK2、STAT3 mRNA表达的影响[J].中华物理医学与康复杂志,2017,39(8):572-576
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| 电针对慢性脑低灌注大鼠认知功能及海马JAK2、STAT3 mRNA表达的影响 |
| Electroacupuncture can improve cognition in those with chronic cerebral hypoperfusion |
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| DOI： |
| 中文关键词: 电针 慢性脑低灌注 认知功能 JAK2/STAT3 |
| 英文关键词: Electroacupuncture Cerebral hypoperfusion Cognition JAK2/STAT3 mRNA |
| 基金项目:国家自然科学基金项目（81503645）；浙江省重点科技创新团队计划项目（2013TD15） |
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| 中文摘要: |
| 目的 探讨电针对慢性脑低灌注（CCH）大鼠认知功能及海马JAK2、STAT3 mRNA表达水平的影响。 方法 选取清洁级健康雄性SD大鼠40只，分为假手术组10只、手术组30只，手术组采用双侧颈总动脉结扎法制作CCH模型，再随机分为模型组、电针1周组、电针4周组3个亚组，各亚组均为10只。电针4周组采用2/100 Hz疏密波电针连续干预4周，电针1周组仅在评定前最后1周采用电针干预。采用Morris水迷宫系统评定各组大鼠的认知功能，采用逆转录-聚合酶链式反应（RT-PCR）检测JAK2 mRNA、STAT3 mRNA的表达水平。 结果 与假手术组比较，手术组BCCAO术后局灶性脑血流水平显著下降，且低于组内手术前（P<0.05）。与模型组比较，电针4周组第3天开始，逃避潜伏期改善，差异有统计学意义（P<0.05），电针1周组第5天逃避潜伏期显著改善，差异有统计学意义（P<0.05），电针4周组目标象限停留时间明显延长，差异有统计学意义（P<0.05），电针1周组、电针4周组JAK2/STAT3 mRNA表达水平均下降，电针4周组明显下降，差异有统计学意义（P<0.05）。与电针1周组比较，电针4周组第5天逃避潜伏期改善（P<0.05），目标象限停留时间延长（P<0.05），JAK2 mRNA下降更明显（P<0.05）。 结论 电针能改善CCH大鼠的认知功能，早期干预作用更显著，其机制可能是通过抑制脑低灌注后JAK2/STAT3通路的过度活化，进而减轻脑损伤。 |
| 英文摘要: |
| Objective To investigate the effect of electroacupuncture (EA) on the cognitive function of rats with chronic cerebral hypoperfusion (CCH) and its possible mechanism. Methods Forty male Sprague-Dawley rats were randomly divided into a sham operation group of 10 and an operation group of 30. Permanent bilateral common carotid artery occlusion (BCCAO) was conducted to introduce a model of CCH in the operation group, which was further divided into a model group, an EA 4 w group and an EA 1 w group, each of 10. The EA 4 w group was given 2/100 Hz dilatational wave EA for 4 weeks, and the EA 1 w group was provided with the same EA for one week. A Morris water maze was used to assess the rats′ cognitive functioning, and a reverse transcription polymerase chain reaction (RT-PCR) was employed to detect the expression of JAK2/STAT3 mRNA in all the rats′ brains. Results Compared with the sham operation group, the rCBF in the operation group had decreased significantly after BCCAO, and it was significantly lower than before the operation. Compared with the model group, the escape latency improved significantly from the 3rd day in the EA 4 w group, and from the 5th day in the EA 1 w group. The targeted quadrant residence time of the EA 4 w group was prolonged significantly. JAK2/STAT3 mRNA expression decreased significantly only in the EA 4 w group. Compared with the EA 1 w group, the escape latency of the EA 4 w group had decreased significantly by the 5th day, with the target quadrant residence time significantly prolonged, and JAK2 mRNA had decreased significantly as well. Conclusion EA can improve cognition in CCH, at least in rats, and especially with early intervention. This may be achieved through inhibiting excessive activation of the JAK2/STAT3 pathway, reducing brain injury. |
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