汤翔宇，付涛，刘阳，刘朝旭.1.0 mT低频交变电磁场对大鼠骨髓间充质干细胞增殖及成骨分化的影响[J].中华物理医学与康复杂志,2017,39(8):566-571
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| 1.0 mT低频交变电磁场对大鼠骨髓间充质干细胞增殖及成骨分化的影响 |
| Effects of an electromagnetic field on the proliferation and osteogenic differentiation of bone mesenchymal stem cells |
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| DOI： |
| 中文关键词: 电磁场 干细胞 增殖 成骨分化 矿化 |
| 英文关键词: Electromagnetic fields Stem cells Proliferation Osteogenic differentiation Mineralization |
| 基金项目:国家自然科学基金项目（31300799，51537004）；华中科技大学自主创新基金项目（2014QN094） |
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| 中文摘要: |
| 目的 探讨1.0 mT低频交变电磁场对大鼠骨髓间充质干细胞活力、增殖及成骨分化的影响。 方法 本研究选用1.0 mT正弦波电磁场进行实验，电磁场频率分别设置为10 Hz，30 Hz，50 Hz和70 Hz。不同频率组大鼠骨髓间充质干细胞每天均接受8 h暴磁处理，培养时间为2周。分别在不同时间点使用Live/Dead试剂盒检测不同频率电磁场对细胞活力的影响；使用DNA定量方法评价细胞增殖水平；使用Von Kossa染色方法检测细胞外基质矿化程度；并使用酶联免疫吸附和免疫组化方法检测各组骨钙素和骨形态发生蛋白-2合成情况。 结果 实验结果显示，50 Hz及70 Hz电磁场对大鼠骨髓间充质干细胞活力有明显影响；10 Hz电磁场可显著促进细胞增殖；暴磁2周后，发现50 Hz组骨钙素及骨形态发生蛋白合成水平明显高于其它暴磁组；并且50 Hz组中矿化结节数量及密度也较其它组明显增加。 结论 不同频率1.0 mT低频交变电磁场对大鼠骨髓间充质干细胞活力、增殖及成骨分化方面的影响各不相同，本研究结果为电磁场应用于骨组织再生医学提供更多理论依据。 |
| 英文摘要: |
| Objective To explore the effect of an electromagnetic field (EMF) on the viability, proliferation and osteogenic differentiation of rats′ bone mesenchymal stem cells (rBMSC). Methods Sinusoidal 1mT EMFs of 10 Hz, 30 Hz, 50 Hz and 70 Hz were administered to rBMSC for 2 hours per day during 2 weeks of cultivation. The cell viability was analyzed using live/dead assays. The cells′ DNA was quantified to evaluate cell proliferation. Von Kossa staining was performed to study the mineralization of the extracellular matrix. The enzyme linked immunosorbent assay (ELISA) and immunohistological staining were employed to observe the synthesis of osteocalcin (OC) and bone morphogenetic protein-2 (BMP-2). Results Cell viability decreased under exposure to the 50 Hz and 70 Hz fields. The proliferation of rBMSC was elevated significantly in the 10 Hz EMF-treated group. The expression OC and BMP-2 was elevated after two weeks of 50 Hz EMF stimulation. Moreover, the mineralization of the extracellular matrix was found to be enhanced after 50 Hz EMF exposure. Conclusion Exposure to a 1 mT EMF has different effects on the viability, proliferation and osteogenic differentiation of rBMSC depending on the frequency. The data provide practical guidance for applying electromagnetic fields in bone regenerative medicine. |
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