孟庆楠，王益民，阎冰，马轶文，岳冬梅.不同磁感强度磁场对人脑微血管内皮细胞生物学效应的影响[J].中华物理医学与康复杂志,2017,39(11):801-805
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| 不同磁感强度磁场对人脑微血管内皮细胞生物学效应的影响 |
| The biological effects of magnetic fields on microvascular endothelial cells in the human brain |
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| DOI： |
| 中文关键词: 磁感强度 人脑微血管内皮细胞 乳酸脱氢酶 氧化损伤 细胞凋亡 细胞形态 |
| 英文关键词: Magnetic induction Human brain microvascular endothelial cells Oxidative damage Cell apoptosis Cell morphology |
| 基金项目:国家自然科学基金资助项目(30873467) |
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| 中文摘要: |
| 目的 观察不同磁感强度磁场对人脑微血管内皮细胞（HBMEC）生物学效应的影响。 方法 提取传代后处于对数生长期的HBMEC并将其分为对照组、不同强度（其极面中心磁感强度分别为26mT、62.5mT、110.7mT、215.6mT)磁场组。经体外培养72h后，分别采用乳酸脱氢酶（LDH）法检测各组细胞细胞膜改变，采用超氧化物歧化酶（SOD）法、丙二醛（MDA）法检测磁场对细胞氧化损伤的影响；采用倒置显微镜观察细胞形态学变化；采用流式细胞仪观察细胞凋亡情况。 结果 与对照组比较，磁感强度为215.6mT的磁场组细胞LDH值明显升高，组间差异具有统计学意义（P<0.05），其细胞氧化损伤、凋亡情况与对照组间差异无统计学意义（P>0.05），细胞形态也无明显改变；与对照组比较，磁感强度26mT、62.5mT、110.7mT的磁场组细胞LDH值、细胞氧化损伤及凋亡均无明显差异（P>0.05），细胞形态无明显改变。 结论 不同磁感强度磁场对HBMEC生物学效应的影响不同，其中磁感强度215.6mT的磁场对HBMEC细胞膜具有一定影响作用，但对细胞氧化损伤、凋亡及形态尚无明显影响，其作用效果及相关机制还有待进一步探讨。 |
| 英文摘要: |
| Objective To observe the biological effects of magnetic fields of different intensities on microvascular endothelial cells in the human brain (HBMECs). Methods HBMECs were cultured in vitro under normal conditions and randomly divided into a control group and several magnetic induction groups——26 mT, 62.5 mT, 110.7 mT and 215.6 mT at the center pole. Any changes to the cytomembranes were observed 72 h after planking using the lactate dehydrogenase (LDH) method. Superoxide dismutase and malondialdehyde methods were used to detect cellular oxidation due to the magnetic field. An inverted microscope was used to observe any changes in cell morphology, and flow cytometry was employed to detect cell apoptosis. Results Compared with the control group, the LDH value of the 215.6 mT group was significantly higher, but there were no significant differences in oxidative damage, apoptosis or morphology observed. Moreover, there were no significant differences between the controls and the 26 mT, 62.5 mT and 110.7 mT groups in any of the above measurements. Conclusion Magnetic fields of different intensity have different biological effects on HBMECs. A 215.6 mT magnetic field influences their cell membranes but causes no oxidative damage, cell apoptosis or morphological changes. These observations and the mechanism need further exploration. |
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