李哲，范家宏，郭钢花，关晨霞，乐琳，郭君.磁刺激诱导下高迁移率族蛋白B1对星形胶质细胞迁移能力的影响[J].中华物理医学与康复杂志,2017,39(10):721-726
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| 磁刺激诱导下高迁移率族蛋白B1对星形胶质细胞迁移能力的影响 |
| The role of high-mobility group box 1 in magnetic stimulation-induced migration of astrocytes |
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| DOI： |
| 中文关键词: 磁刺激 星形胶质细胞 HMGB1 细胞迁移 |
| 英文关键词: Magnetic stimulation Astrocytes Box 1 Migration |
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| 中文摘要: |
| 目的 探讨磁刺激诱导星形胶质细胞迁移的机制及高迁移率族蛋白B1（HMGB1）对星形胶质细胞迁移的影响。 方法 取新生2～3d SD大鼠大脑皮质星形胶质细胞，用细胞免疫荧光法检测细胞纯度；将细胞分为磁刺激组和对照组，磁刺激组给予磁刺激，对照组在相同环境下，不给予磁刺激。将细胞分为实验组和控制组，实验组用10μmol/ml U0126抑制剂预刺激30min，控制组不加入U0126抑制剂，以探讨细胞外信号调节激酶（ERK1/2）与HMGB1的关系；将细胞分为siRNA转染组和HMGB1-siRNA转染组，SiRNA组给予HMGB1 siRNA处理，以探讨HMGB1对细胞迁移能力的影响。用Western blot检测磁刺激对HMGB1和ERK1/2的影响，用划痕实验检测磁刺激对星形胶质细胞迁移能力的影响。 结果 10Hz磁刺激可促进ERK磷酸化，增强星形胶质细胞的迁移能力，并可增加HMGB1的表达水平；用U0126试剂处理后，HMGB1的表达量明显下降；HMGB1 siRNA转染后HMGB1表达量明显下降，且星形胶质细胞的迁移能力显著下降。 结论 星形胶质细胞在磁刺激作用下，通过激活ERK通道，促进ERK磷酸化，增加下游HMGB1的表达水平，且HMGB1以自分泌的形式促进自身迁移。 |
| 英文摘要: |
| Objective To examine the molecular mechanism involved in astrocyte migration induced by magnetic stimulation (MS), and the role of high mobility group box 1 (HMGB1) in migration. Methods Astrocytes were isolated from the cortical tissues of 2-3 day old Sprague-Dawley rats and divided into a stimulus group given MS and a control group without MS. The stimulus group was further divided into an experimental group and a control group, with the former pre-stimulated with 10 μmol/ml U0126 for 30 minutes and no pre-stimulation for the latter.The cells in the experimental group were also randomly divided into siRNA and HMGB1-siRNA transfection groups to examine the role of HMGB1 in astrocyte migration induced by MS. The SiRNA group was transfected with HMGB1 siRNA. Western blotting was used to detect any effect of MS on HMGB1 and extracellular signal-regulated kinase1/2 (ERK1/2) .The migration of astrocytes was detected using the scratch assay. Results MS (10 Hz) can promote the phosphorylation of ERK, increase the migration of astrocytes and the expression of HMGB1. After the U0126 treatment and transfection with HMGB1 siRNA, the effects of MS on expression of HMGB1 and migration of astrocytes decreased significantly. Conclusions Magnetic stimulation-mediated migration of astrocytes via activation of the ERK pathway phosphorylation and autocrine of HMGB1. |
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