赵梅兰，曹晓哲，王德文，刘杰，哈小琴，吴丹莉，张燕.电磁脉冲辐射对原癌基因c-fos调控区的影响[J].中华物理医学与康复杂志,2004,(2):
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| 电磁脉冲辐射对原癌基因c-fos调控区的影响 |
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| DOI： |
| 中文关键词: 电磁脉冲 c-fos基因 P500-tkCAT P350-tkCAT P250-tkCAT |
| 英文关键词: Electromagnetic pulse c-fos gene P500-tkCAT P350-tkCAT P250-tkCAT |
| 基金项目:解放军“十五”指令课题基金资助（No.01L023） |
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| 中文摘要: |
| 目的通过研究电磁脉冲（electromagnetic pulse，EMP）辐射对原癌基因c-fos调控区域的影响，探讨其诱导细胞功能改变的机制。 方法构建c-fos启动区氯霉素乙酰化转移酶（chloramphenicol acetyl-transferase，CAT），然后转染HeLa癌细胞株，高场强EMP模拟源(有界波模拟源)辐射细胞，EMP场强为6×104 V/m，脉冲上升时间为20 ns，脉宽为30 μs,频率为2.5个脉冲/min，辐射2 min，处理后20 min时观察细胞中 CAT的活性。 结果EMP辐射转染的细胞后20 min，转染了P500（-711～-223 bp）和P250（-362～-100）的Hela细胞其CAT活性明显高于对照水平的活性（P<0.01）。 结论EMP辐射可引起c-fos基因调控区的-362～-225明显改变。 |
| 英文摘要: |
| Objective To determine whether regulatory regions of c-fos gene were responsive to electromagnetic pulse (EMP) and to explore the possible underlying mechanisms. MethodsHela cells were transiently transfected with plasmids containing upstream regulating regions of c-fos up to -700bp, coupled with the prokaryotic reporter gene CAT（chloramphenical acetyl-transferase）. Cells were exposed to EMP（simulator which provides 5 pulses/2 min with a high electric field intensity 60kV/m, 20-nsec rise time and 30ms pulse width.）. CAT was measured by enzymatic determinations. Acetylated 14C-chlormphenicol was measured by thin layer chromatography. ResultsCAT expression above control levels in transfected cells (region -362～-225bp) was observed at 20 min following exposure to EMP，especially in P500（-711～-223bp）and P250（-362～-100）group(P<0.01). ConclusionEMP affects the regulation regions of c-fos gene. |
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