葛保健，方真华，赵文春，任凯，冷燕，奎吴华.工频电磁场对小鼠骨髓间充质干细胞BMP-2和TGF-β1mRNA表达的影响[J].中华物理医学与康复杂志,2004,(5):
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| 工频电磁场对小鼠骨髓间充质干细胞BMP-2和TGF-β1mRNA表达的影响 |
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| DOI： |
| 中文关键词: 骨髓间充质干细胞 骨生长因子 逆转录PCR 电磁场 |
| 英文关键词: Bone marrow mesenchymal stem cell Skeletal growth factor RT-PCR Electromagnetic fields |
| 基金项目:国家自然科学基金主任基金（No.50347025） |
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| 中文摘要: |
| 目的研究工频电磁场对体外培养的小鼠骨髓间充质干细胞BMP-2和TGF-β1 mRNA表达的影响。 方法体外分离培养小鼠骨髓间充质干细胞，取第2代细胞分组暴磁，然后用RT-PCR技术分别检测各组细胞的BMP-2和TGF-β1 mRNA表达，并进行定量分析。 结果适当强度及作用时间的工频电磁场刺激可使小鼠骨髓间充质干细胞mRNA表达明显增强。相同作用时间下，BMP-2 mRNA的表达于1.6 mT场强中达最大值，而TGF-β1mRNA的表达则于0.4 mT场强中达最大值；1.6 mT场强条件下，电磁场连续照射5 d，每日1 h，可使BMP-2和TGF-β1mRNA表达均达到最大值。 结论适当“窗口”的电磁场刺激对体外培养的小鼠骨髓间充质干细胞BMP-2和TGF-β1mRNA表达有明显的促进作用。 |
| 英文摘要: |
| Objective To study the effects of 50Hz electromagnetic field (EMF) on mRNA expression of BMP-2 and TGF-β1 in mouse bone marrow mesenchymal stem cells (MSCs) in vitro. Methodsthe mouse bone marrow mesenchymal stem cells were isolated and cultured in vitro. The second-passage cells were harvested and divided into different groups. 50Hz EMF stimulation was conducted accordingly. The expression of mRNA of BMP-2 and TGF-β1 in the cells in every group was examined by reverse-transcriptase polymerase chain reaction (RT-PCR). ResultsVarious intensities and times of exposure of 50Hz EMF induced a significant increase of BMP-2 and TGF-β1 mRNA in comparison to the controls. The expression of BMP-2 mRNA peaked with the stimulation intensity of 1.6mT. The corresponding intensity for TGF-β1 peak expression was 0.4mT. Both BMP-2 expression and TGF-β1 mRNA expression were increased to peak in cells when exposed continuously for 60 minutes per day for 5 days. ConclusionStimulation of 50Hz EMF can increase significantly the expression of BMP-2 and TGF-β1mRNA in mouse bone marrow mesenchymal stem cells in vitro. |
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