王涛，郭铁成，朱晓临，张扬，张允健，孙圣刚，梅元武.重复经颅磁刺激治疗脑出血家兔的实验研究[J].中华物理医学与康复杂志,2003,(7):.-
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| 重复经颅磁刺激治疗脑出血家兔的实验研究 |
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| DOI： |
| 中文关键词: 经颅磁刺激 脑出血 细胞凋亡 细胞因子 家兔 |
| 英文关键词: Repetitive transcranial magnetic stimulation(rTMS) Cerebral hemorrhage Apoptosis Cytokine Rabbit |
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| 中文摘要: |
| 目的探讨重复经颅磁刺激(rTMS)对脑出血家兔的治疗作用及其相关机制。 方法家兔36只，随机分为A、B、C组。A组（治疗组）和B组（模型组）采用家兔自体血注射制作脑出血模型，C组（对照组）注射生理盐水。A组家兔于造模后12 h开始实施rTMS，每日3次；B组和C组不进行rTMS。各组家兔分别于模型制作后12 h、24 h、48 h、72 h、1周、2周处死。采用TUNEL法和免疫组化技术，检测出血灶周围脑组织中细胞凋亡及bcl-2、TNF-α和IL-6的表达。 结果3组均未见自发性出血。C组未见凋亡细胞及bcl-2阳性表达；A组和B组均有凋亡细胞和bcl-2表达水平增高，2组相比，A组的凋亡细胞较少，bcl-2表达水平更高（P＜0.05）。C组无TNF-α和IL-6阳性细胞；A组和B组均有TNF-α和IL-6表达，2组相比，A组的TNF-α表达水平较低，IL-6表达较高（P均＜0.05）。 结论rTMS刺激可通过调控炎症因子表达、减轻炎性损伤以及上调bcl-2的表达、减少细胞凋亡，在脑出血中发挥治疗作用。 |
| 英文摘要: |
| Objective To observe the possible therapeutic effect of repetitive transcranial magnetic stimulation(rTMS) on cerebral hemorrhage in rabbits and explore the underlying mechanism. MethodsThirty-six rabbits were divided randomly into 3 groups, group A (receiving rTMS) and group B (no rTMS) were experimental groups, and group C was control group. The cerebral hemorrhage model was made by injection of self-body blood, and the control group was made by injection of saline. The rabbits in group A were treated with rTMS 3 times a day and those in the groups B and C without rTMS. Rabbits in all the 3 groups were sacrificed separately after 12, 24, 48, 72 hours and 1 and 2 weeks after the establishment of the model. TUNEL and immunohistochemistry methods were used to detect the apoptosis and the expressions of TNF-α, bcl-2 and IL-6 in the perihematomal brain tissues. Results No spontaneous intracerebral hemorrhage was observed in all of the 3 groups. Neither positive apoptotic cell and bcl-2 expression nor expressions of TNF-α and IL-6 was observed in group C. The apoptotic cells were found in both groups A and B, with less of the cells in group A than those in group B(P＜0.05). The increased expressions of bcl-2, TNF-α and IL-6 were detected in both groups A and B. Compared to group B, the levels of bcl-2 and IL-6 were significantly higher in group A (P＜0.05), and in contrast, the expressions of TNF-α was reduced to a significant extent in group A after rTMS (P＜0.05). Conclusion rTMS could be beneficial for treating cerebral hemorrhage by regulating the expressions of the inflammatory cytokines such as TNF-α and IL-6, diminishing the inflammatory lesions, and reducing the apoptosis course by up-regulating the expression of bcl-2. |
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