王健瑞，乔晋琳，路平，邓轶，刘凤桐，车向宜，董兆德，陈蕾，马广昊.第三腰椎横突综合征动物实验模型的建立及实验研究[J].中华物理医学与康复杂志,2003,(7):.-
扫码阅读全文
|
| 第三腰椎横突综合征动物实验模型的建立及实验研究 |
|
| |
| DOI： |
| 中文关键词: 腰椎 横突综合征 炎症 损伤 动物模型 |
| 英文关键词: Transverse process syndrome Lumbar vertebra Animal model |
| 基金项目:中国人民解放军海军后勤部资助课题 |
|
| 摘要点击次数: 3161 |
| 全文下载次数: 3320 |
| 中文摘要: |
| 目的通过模拟第三腰椎横突周围无菌性炎症的方法，建立第三腰椎横突综合征动物实验模型，对两种不同动物重复用同一造模方法以考察其可靠性。 方法将90只SD大鼠及20只日本大耳白纯种兔分别随机分为4组：正常组、假手术组、材料实验组及模型组。分别于造模后4，10和30 d进行组织病理学观察；同时进行全血、前列腺环素、内皮素、5-羟色胺观察；在0，1，8和15 d对大鼠进行机械性压迫痛阈实验。 结果本模型可以在第三腰椎横突周围出现明显无菌性炎症、瘀血、水肿及后期有瘢痕形成。经组织细菌培养，无细菌感染。模型组炎性介质等指标显著高于材料实验组、假手术组及正常组（P<0.01）。 结论本模型可以对第三腰椎横突周围无菌性炎症作出真实反映。 |
| 英文摘要: |
| Objective To establish the experimental models of transverse process of the third lumbar vertebra syndrome in rats and rabbits with the aim of understanding its pathophysiological mechanisms. MethodsNinety Sprague-Dawley rats and 20 rabbits were randomly divided into 4 groups, respectively: (1) the model group, in which a 0.5mm×0.5mm gelfoam was prepared and inserted into the deep subfascial part of the 3rd lumbar transverse process; (2) the sham operation group, in which the operative process was only for exposure of the 3rd transverse process; (3) the operative process was about the same as the model group, while the gelfoam was only placed under the gluteus maximus;(4) the normal group, in which no surgical intervention was administered. The animals were sacrificed on the 4th , 10th and 30th day post-operation, and pathological observations were carried out, respectively, and the blood platelets, ET-1, PGI2, 5-HT contents were measured simultaneously; and pain threshold was determined with mechanical compression method on the 0th, 1st, 8th anf 15th days post-operation. Results In the model group, characteristic aseptic inflammatory changes could be observed around the 3rd transverse process. All the parameters denoting inflammatory changes were significantly higher than that of other 3 groups (P<0.01). Conclusion This animal model can well reflect the aseptic inflammatory changes commonly found in patient with the transverse process of the 3rd lumbar vertebra syndrome. |
|
查看全文
查看/发表评论 下载PDF阅读器 |
| 关闭 |
|
|
|
