张大伟、王强、马健、柏广涛、高晶晶、李明、宫阳阳.强化训练对脑卒中后抑郁大鼠抑郁程度及海马区Kalirin-7表达的影响[J].中华物理医学与康复杂志,2016,38(4):241-245
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| 强化训练对脑卒中后抑郁大鼠抑郁程度及海马区Kalirin-7表达的影响 |
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| DOI： |
| 中文关键词: 强化训练 脑卒中 抑郁 |
| 英文关键词: Intensive training Stroke Post-stroke depression |
| 基金项目:山东省自然科学基金（ZR2104JL057） |
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| 中文摘要: |
| 目的 观察强化训练对脑卒中后抑郁大鼠抑郁程度及海马区Kalirin-7表达的影响，探讨强化训练改善卒中后抑郁大鼠抑郁程度可能的机制。 方法 取40只雄性Wistar，采用随机数字法将大鼠分为假手术组、脑卒中后抑郁（PSD）组、普通训练组、强化训练组，每组10只。PSD组、普通训练组、强化训练组采用线栓法建立左侧大脑中动脉（MCAO）模型，并给予慢性不可预知性应激刺激(CMS)，假手术组仅分离颈总、颈内、颈外动脉，不做插线处理，也不给予CMS。造模成功后，假手术组与PSD组不进行跑台训练，普通训练组、强化训练组分别进行普通和强化跑台训练。4组大鼠分别于训练前和训练14d、28d后进行行为学实验［糖水消耗实验（SPT）、旷场试验（OFT）、强迫游泳实验（FST）］，然后采用western blot法检测其左侧海马区Kalirin-7蛋白的表达情况。 结果 训练14d后，PSD组、普通训练组、强化训练组各项行为学实验结果与假手术组相比，差异均有统计学意义（P<0.05）,普通训练组的OFT和FST结果分别为（55.71±3.94）s和（67.42±3.64）s，与PSD组相比，差异均有统计学意义（P<0.05）,强化训练组各项行为学实验结果与PSD组和普通训练组相比，差异均有统计学意义（P<0.05）;训练28d后，PSD组、普通训练组各项行为学实验结果与假手术组相比，差异均有统计学意义（P<0.05），普通训练组和强化训练组各项行为学实验结果与PSD组相比，差异均有统计学意义（P<0.05），且强化训练组各项行为学实验结果与普通训练组相比，差异均有统计学意义（P<0.05）。Western Blot检测显示，普通训练组、强化训练组海马区Kalirin-7的表达均显著高于PSD组同时间点（P<0.05）；强化训练组海马区Kalirin-7表达显著高于普通训练组同时间点（P<0.05）。 结论 强化训练可改善脑卒中后抑郁大鼠的抑郁程度，增加海马区Kalirin-7的表达，促进其神经功能的恢复，并且强化训练的效果优于普通训练。 |
| 英文摘要: |
| Objective To observe any effect of intensive training on the degree of depression and the expression of Kalirin-7 in the hippocampus of rats modeling with post-stroke depression, and to investigate possible mechanisms. Methods Forty male Wistar rats were randomly divided into a post-stroke depression (PSD) group, a routine training group, an intensive training group and a sham-operation group (n=10 for each group). All except the sham-operation group were given the middle cerebral artery occlusion using the intraluminal thread method, and chronic mild stress was applied. The PSD group and the sham-operation group were not given any training. The routine training group was trained once a day, and the intensive training group was trained twice a day. The sucrose preference test, open field test and forced swimming test were conducted at the outset and after 14 and 28 days of training. The expression of Kalirin-7 in the left hippocampus was detected using Western blotting. Results After 14 days of training, significant differences were found in the test results of the sham-operation group compared with the other groups (P<0.05). There were also significant differences in the average results on all 3 tests among all of the groups except the sham-operation group. At both 14 and 28 days after training, the average expression of Kalirin-7 in both the routine and intensive training groups was significantly higher than in the PSD group (P<0.05), and that of the intensive training group was significantly higher than that of the routine training group at the same time points (P<0.05). Conclusion Intensive training can relieve depression and increase the expression of the Kalirin-7 in the hippocampus, at least in rats. |
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