孙泊，庄涛.运动训练对大鼠心肌内皮素含量及受体分型的影响[J].中华物理医学与康复杂志,2006,(11):
扫码阅读全文
|
| 运动训练对大鼠心肌内皮素含量及受体分型的影响 |
|
| |
| DOI： |
| 中文关键词: 运动训练 内皮素-1 内皮素受体 心肌 |
| 英文关键词: Sports training Endothelin-1 Endothelin receptor Myocardium |
| 基金项目: |
|
| 摘要点击次数: 1936 |
| 全文下载次数: 2794 |
| 中文摘要: |
| 目的探讨运动训练对大鼠心肌组织内皮素-1（ET-1）含量、ET受体分型及受体特征的影响。 方法选用健康雄性SD大鼠进行跑台运动训练，采用放射免疫法测定大鼠心肌ET-1含量，采用放射性配基结合受体分析法测定心肌组织ETAR及ETBR的变化情况。 结果大鼠经运动训练后，其心肌细胞膜的ET-1含量下降(P<0.01)，心肌细胞膜ETAR最大结合量降低（P<0.05），解离常数Kd值无显著改变；ETBR的最大结合容量升高（P<0.01），解离常数Kd值显著降低(P<0.01)。 结论运动训练主要通过调节ET不同亚型受体的比例、亲和力及结合容量，从而对ET-1的生成、清除及与受体的结合能力产生影响，使ET的作用效力发生改变，从而增强心脏的调节功能。 |
| 英文摘要: |
| Objective To explore the effects of sports training on endothelin-1 （ET-1） and endothelin-1 receptor （ETR） and its subtypes in the myocardium in rats. MethodsAfter sports training, levels of endothelin-1 were measured using radioimmunoassay, and changes in ETAR and ETBR levels by radioligand binding assay. ResultsThe endothelin-1 level in myocardial cells was significantly reduced (P<0.01) in the trained group, the maximal binding capacity (Bmax) of ETAR was reduced （P<0.05）, the dissociation constant (KD) was unchanged, the Bmax of ETBR increased (P<0.01),and its KD was reduced (P<0.01). ConclusionSports training can change the proportion of different subtypes and activity of ETR in the myocardium, and change the rate of generation and elimination of ET-1 and its ability to combine with its receptors. This influences the effect of ET-1, and improves the regulation of the heart during exercise. |
|
查看全文
查看/发表评论 下载PDF阅读器 |
| 关闭 |
|
|
|
