孙新华，林淑艳，曲冬.氦-氖激光对兔正畸牙移动牙周组织中基质金属蛋白酶-9表达的影响[J].中华物理医学与康复杂志,2004,(3):
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| 氦-氖激光对兔正畸牙移动牙周组织中基质金属蛋白酶-9表达的影响 |
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| DOI： |
| 中文关键词: 氦-氖激光 牙齿移动 牙周组织 基质金属蛋白酶 |
| 英文关键词: He-Ne laser Tooth movement Periodontal tissue Matrix metalloproteinase |
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| 摘要点击次数: 1881 |
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| 中文摘要: |
| 目的研究He-Ne激光照射对兔实验性牙移动牙周组织中基质金属蛋白酶-9（MMP-9）表达的影响，探讨He-Ne激光局部照射对促进正畸牙齿移动及牙周组织改建的可行性。 方法30只大耳白兔，随机分为6组，每组5只，其中实验动物右侧为照射侧，左侧为对照侧。各组动物分别经正畸实验装置给予持续1，3，5，7，14及21 d的加力处理，同时实验动物右侧局部牙周组织给予He-Ne激光照射，左侧则不进行特殊处理。各组实验动物分别于实验结束后处死，对实验样本进行MMP-9免疫组织化学染色、图像分析，观察兔实验性牙移动牙周组织中MMP-9表达与分布的变化，并进行统计学分析。 结果He-Ne激光照射侧压力区与张力区MMP-9的表达增强现象均较对照侧出现得早，且峰值亦较对照侧高。张力区兔牙周组织中MMP-9表达灰度分析表明，术后第5，7天时，照射侧与对照侧间差异有显著性意义（P<0.05），压力区兔牙周组织中MMP-9表达灰度分析表明，术后第3，5，7天时，照射侧与对照侧间差异有显著性意义（P<0.05或0.01）。 结论He-Ne激光照射增强了兔正畸牙周组织中MMP-9的表达，提示He-Ne激光照射促进了正畸牙移动过程中的血管化反应及骨改建过程。 |
| 英文摘要: |
| Objective To explore the feasibility of He-Ne laser irradiation in promoting orthodontic tooth movement and periodontal remodeling through observing the effect of low power laser on the expression and distribution of matrix metalloproteinases-9(MMP-9) during orthodontic tooth movement in rabbit. MethodsThirty rabbits were divided into 1d，3d，5d，7d，14d and 21d groups, each group were given a coil spring connected to the maxillary upper first molar to the upper incisor and fixed bilaterally by a ligature wire and set force to 80g. He-Ne laser irradiation was performed on the right side of the orthodontic tooth, and the left side served as control. Radiation time ranged from 1 day to 5 days, once a day, 15min each time. All of the tissue sections were proceeded with MMP-9 immunohistochemical staining. MMP-9 average gray scale in periodontal tissue of the orthodontic teeth was analyzed by a Computer Image Analyzing System. ResultsThe expression level of MMP-9 increased earlier and lasted for a longer time in the compression and tension area on the side of irradiation by He-Ne laser as compared to those on the control side. There was significant difference between the irradiation side and the control side in terms of the MMP-9 expression gray scale in the tension zones at 5 and 7 days post-operation, and in the pressure zones at 3,5,7 day post-operation. ConclusionDuring the movement of orthodontic teeth, local irradiation with He-Ne laser could increase the expression of MMP-9. It was suggested that the irradiation with low power laser accelerate blood vessel and bone remodeling. |
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