吴伟，汪洋，邱阳.高压氧对持续局灶性脑缺血细胞凋亡的影响及其作用机制[J].中华物理医学与康复杂志,2003,(8):.-
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| 高压氧对持续局灶性脑缺血细胞凋亡的影响及其作用机制 |
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| DOI： |
| 中文关键词: 局灶脑缺血 细胞凋亡 高压氧治疗 |
| 英文关键词: Focal cerebral ischemia Apoptosis Hyperbaric oxygenation |
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| 中文摘要: |
| 目的探讨高压氧（HBO）对持续性局灶性脑缺血细胞凋亡的影响及其作用机制。 方法选择4~5个月龄SD大鼠65只，随机分为缺血组、HBO组和假手术组。观察脑梗死灶体积、凋亡细胞数量及凋亡调控基因蛋白表达的变化。 结果HBO组与缺血组相比，6 h~10 d各相应时间点梗死灶体积均明显缩小，差异均有极显著性意义（P均<0.01）；HBO组缺血48 h后，TUNEL阳性凋亡细胞数较缺血组明显减少（P<0.01）；HBO组与缺血组相比，在缺血6 h~10 d各时间点Bcl-2阳性细胞增加，差异有显著性意义；Bax阳性细胞在缺血6 h~10 d各时间点均减少，差异有显著性意义。 结论脑缺血早期暴露于高压氧可以减少局灶性脑缺血梗死灶体积；高压氧能抑制脑缺血细胞凋亡，其作用与上调Bcl-2蛋白、下调Bax蛋白表达有关。 |
| 英文摘要: |
| Objective To study the effect of hyperbaric oxygenation （HBO） on apoptosis in focal cerebral ischemia and the mechanisms underlying it. MethodsSixty five 4-5-month-old Spraque-Dawley（SD）rats were randomly divided into three groups: the focal cerebral ischemia group，FCI group，HBO group and the control group.The focal brain tissue infarct sizes（BTIS），the number of apoptotic cells and the expression of bcl-2 and Bax protein were examined at different time points. ResultsThe BTIS were significantly reduced from 6 hours to 10 days, the number of apoptotic cells were significantly decreased in 48 hours after HBO in the HBO group （P<0.01）. The bcl-2 protein levels were increased significantly and Bax protein levels decreased significantly in HBO group （all P<0.01）. ConclusionHBO can reduce focal brain tissue infarct size and inhibit apoptosis when administered in early stage of focal cerebral ischemia. This may be related to the up-regulation of bcl-2 protein levels and down-regulation of Bax protein levels. |
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