刘芳，黄海龙，陈红，侯丽敏，邓雨果，尤春景.局灶性脑缺血成年大鼠高压氧干预后神经干细胞增殖及分化研究[J].中华物理医学与康复杂志,2015,(10):733-738
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| 局灶性脑缺血成年大鼠高压氧干预后神经干细胞增殖及分化研究 |
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| DOI： |
| 中文关键词: 高压氧 局灶性脑缺血 神经干细胞 增殖 神经元 |
| 英文关键词: Hyperbaric oxygen Focal cerebral ischemia Neural stem cells Proliferation Neuron |
| 基金项目:国家自然科学基金（81070986） |
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| 摘要点击次数: 2826 |
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| 中文摘要: |
| 目的观察高压氧（HBO）干预对成年大鼠急性局灶性脑缺血后海马齿状回颗粒下区（SGZ）神经干细胞增殖及向神经元分化的影响。 方法选取48只成年雄性SD大鼠，按随机数字表法分为模型组、高压氧组、高压空气组和常压氧组，每组12只。各组大鼠均进行线栓法MCAO制模，除模型组制模后不接受任何干预外，其余3组均于线栓插入后2h分别给予高压氧，高压空气和常压氧干预，每日1次。采用免疫荧光双重标记制模成功后第2、3、7和14天脑梗死侧SGZ区增殖的神经干细胞（BrdU+/nestin+）及其分化的神经元（BrdU+/DCX+），并在荧光显微镜下计数。 结果制模成功后第2天，高压氧组SGZ区BrdU/nestin和BrdU/DCX的共标细胞数分别为（2340．45±1109.59）个和(5520.66±1103.09)个,分别与常压氧组和模型组同时间点相同细胞比较，差异均有统计学意义（P<0.05）；制模成功后第3和第7天，高压氧组的BrdU/nestin和BrdU/DCX共标细胞数均显著高于高压空气组、常压氧组和模型组，差异均有统计学意义（P<0.05）,且制模成功后第14天,高压氧组的BrdU/DCX共标细胞数亦显著高于高压空气组、常压氧组和模型组，差异均有统计学意义（P<0.05）。 结论高压氧干预可显著促进成年大鼠梗死侧SGZ区神经干细胞的增殖和向神经元分化。 |
| 英文摘要: |
| ObjectiveTo investigate the effect of hyperbaric oxygen intervention at different time on the neural stem cell proliferation and differentiation in dentate gyrus subgranular zone (SGZ) of adult rats after acute focal cerebral ischemia. MethodsTotally 48 Sprague-Dawley male adult rats were randomly divided into a middle cerebral artery occlusion (MCAO) group, a hyperbaric oxygen group, a hyperbaric air group and a normobaric oxygen group, each of 12. A middle cerebral artery occlusion (MCAO) model was induced to all rats using the modified Zea-Longa′s method of intraluminal filament occlusion, Except the MCAO group, the other 3 groups received corresponding hyperbaric oxygen, hyperbaric air and normobaric oxygen intervention once a day two hours after the suture insertion. The rats were sacrificed for double-label immunofluorescent analysis at 2 days, 3 days, 7 days and 14 days after brain ischemia. BrdU+/nestin+ labeled the proliferated neural stem cells, and BrdU+/DCX+ labeled its differentiated derivates, early neurons, in SGZ of ischemic hippocampus dentate gyrus. Also, the cell number was calculated under the fluorescence microscope. ResultsTwo days after brain ischemia, the numbers of BrdU/nestin and BrdU/DCX cells in SGZ in the hyperbaric oxygen group were (2340．45±1109.59) and (5520.66±1103.09) respectively, which had increased significantly, compared with the hyperbaric air group and normobaric oxygen group (P<0.05). Three and 7 days after brain ischemia, the numbers of BrdU/nestin and BrdU/DCX cells in SGZ in the hyperbaric oxygen group had shown significant increase compared with the other 3 groups (P<0.05). Fourteen days after brain ischemia, the numbers of BrdU/DCX cells in SGZ in the hyperbaric oxygen group had significantly increased compared with the hyperbaric air group, normobaric oxygen group and the MCAO group (P<0.05). ConclusionHyperbaric oxygen promotes the proliferation and differentiation of neural stem cells in ischemic SGZ. |
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