刁兰萍，徐建民，吴明，吴直江.激光诱导光动力效应对人癌细胞株及脐血单个核细胞体外作用的比较[J].中华物理医学与康复杂志,2003,(7):.-
扫码阅读全文
|
| 激光诱导光动力效应对人癌细胞株及脐血单个核细胞体外作用的比较 |
|
| |
| DOI： |
| 中文关键词: 血卟啉衍生物 激光 肿瘤细胞 光动力效应 MTT法 净化 |
| 英文关键词: Laser Hematoporphyrin derivative (HPD) Human tumor cell line MTT assay Photodynamic effect |
| 基金项目: |
|
| 摘要点击次数: 3081 |
| 全文下载次数: 3202 |
| 中文摘要: |
| 目的研究激光诱导的光动力效应对人癌细胞株的体外杀伤作用及对脐血单个核细胞的影响，以探讨自体外周血造血干细胞移植物的体外净化方法。 方法通过MTT法测定观察不同剂量光敏剂（血卟啉衍生物，HPD）经激光照射不同时间后,对人白血病细胞株K562、乳腺癌细胞株MCF-7及脐血单个核细胞生长抑制作用的影响。 结果单纯光照对3种细胞均无明显作用；单纯加光敏剂对K562细胞及MCF－7细胞有一定的抑制生长作用；激光照射加光敏剂可显著抑制K562细胞及MCF－7细胞的生长，且具有时间及浓度依赖性，但对脐血单个核细胞则无明显抑制作用。其细胞抑制率依次为K562细胞>MCF－7细胞>脐血单个核细胞。 结论激光诱导的光动力效应可能为恶性血液病或实体瘤患者的自体外周血造血干细胞移植物体外净化提供简便、有效且经济的方法,值得临床推广、应用。 |
| 英文摘要: |
| Objective To investigate the photodynamic effect induced by laser and use it as a method for purging of minimal residual tumor cells from autologous peripheral blood stem cell grafts. MethodsWe studied the effect of various concentrations of hematoporphyrin derivative (HPD) combined with of different time of laser irradiation on human leukemia cell line K562, breast tumor cell line MCF-7 and human umbilical cord blood-derived hematopoietic stem/progenitor cells by using MTT assay to compare the photodynamic effect between cell lines of each group. Results HPD or laser irradiation alone had no apparent cytotoxicity while the application of HPD plus laser exposure caused photosensitization. HPD plus laser irradiation was more efficient for killing K562 cells and MCF-7 cells than doing the normal human umbilical cord blood-derived hematopoietic stem/progenitor cells. The photodynamic effect on K562 cells and MCF-7 cells was positively correlated with the increase of treatment duration and HPD concentration. Conclusion These results indicated that the laser photodynamic effect mediated by HPD might be used for purging autologous peripheral blood stem cell grafts in vitro. |
|
查看全文
查看/发表评论 下载PDF阅读器 |
| 关闭 |
