任凯，吴华，赵文春，葛保健，方真华，印卫锋.工频电磁场对体外培养的小鼠骨髓间充质干细胞增殖与分化的影响[J].中华物理医学与康复杂志,2004,(7):
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| 工频电磁场对体外培养的小鼠骨髓间充质干细胞增殖与分化的影响 |
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| DOI： |
| 中文关键词: 电磁场 环磷酸腺苷 碱性磷酸酶 骨髓间充质干细胞 |
| 英文关键词: Electromagnetic field Cyclic AMP Alkaline phosphatase Bone marrow mesenchymal stem cells |
| 基金项目:国家自然科学基金资助项目（No.50347025） |
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| 中文摘要: |
| 目的研究50 Hz工频电磁场对体外培养的小鼠骨髓间充质干细胞的增殖、分化及细胞内环磷酸腺苷（cAMP）水平的影响。 方法体外培养小鼠骨髓间充质干细胞，取第3代细胞，应用工频电磁场及成骨性诱导剂干预，并分别于第3天和第5天用MTT法检测其增殖活性，用放免法检测细胞内cAMP浓度，于第7天检测细胞内碱性磷酸酶（ALP）的活性。 结果频率50 Hz、强度2 mT的正弦波电磁场能抑制体外培养的小鼠骨髓间充质干细胞的增殖（P<0.05），使其ALP活性增高（P<0.05），在磁场作用的早期（1~3 d），细胞内的cAMP水平明显增高（P<0.01），继续暴磁至第5天，cAMP水平不再发生变化（P>0.05）。 结论50 Hz工频电磁场可能通过作用于cAMP-蛋白激酶A信号转导系统，从而调控体外培养的小鼠骨髓间充质干细胞的增殖与分化，此调控作用可能发生在细胞受电磁场作用的早期。 |
| 英文摘要: |
| Effects of exposure to electromagnetic fields on proliferation and differentiation of mouse bone marrow mesenchymal stem cellsREN Kai，WU Hua，ZHAO Wen-chun，GE Bao-jian，FANG Zhen-hua，YIN Wei-feng．Department of Orthopedics，Tongji hospital，Tongji Medical College，Huazhong University of Science and Technology, Wuhan 430030， China
【Abstract】Objective To study the effects of electromagnetic fields (EMFs) on proliferation, differentiation and intercellular cyclic AMP (cAMP) in mouse bone marrow mesenchymal stem cells (MSCs) in vitro. MethodsThe mouse bone marrow mesenchymal stem cells were isolated and cultured in vitro, the passage three cells were divided into A, B, C and D groups and stimulated with electromagnetic fields (EMFs) under different conditions. The cellular proliferation (MTT), differentiation (alkaline phosphatase activity,ALP), and the intercellular cAMP level were investigated at different time points. ResultsEMFs of 50Hz frequency and 2mT intensity inhibited cellular proliferation (P<0.05), enhanced cellular differentiation (P<0.05), and increased the intercellular cAMP level (P<0.01) in the early stage of the stimulation (1~3 day), the intercellular cAMP level did not change further in the later stage of stimulation. ConclusionLow frequency EMFs might regulate proliferation and differentiation of the mesenchymal stem cells through mediating cAMP-protein kinase A (cAMP-PKA) signal transduction pathway, which occurred in the early period of the stimulation. |
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